Anti-Catenin beta Antibody
Our Anti-Catenin beta sheep polyclonal primary antibody detects human, mouse, and rat Catenin beta , and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.
Product Specifications
Background
Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Short Description
Our Anti-Catenin beta sheep polyclonal primary antibody detects human, mouse, and rat Catenin beta, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.
Synonyms
Catenin beta-1; Beta-catenin; CTNNB1; CTNNB; _-Catenin
UniProt
P35222
Reactivity
Human, Mouse, and Rat
Immunogen
A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Target
Catenin beta
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ICC, IHC-Frozen, WB
Format
Affinity-purified
Buffer
Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Molecular Weight
86-92 kDa
Precautions
For research use only.
Shipping Conditions
25°C (ambient)
Storage Conditions
Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations) . After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
WB Dilution
2 μg/mL
Specificity
The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; .
ICC Dilution
4 μg/mL
Host or Source
Sheep
Physical Properties
Lyophilized
Isotype
IgG
Immunogen Species
Human
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