Anti-pro-Nerve growth factor (proNGF) Antibody

Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.

Our Anti-pro-Nerve growth factor (proNGF) sheep polyclonal primary antibody detects human, mouse, and rat pro-Nerve growth factor (proNGF), and is whole serum. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Maintain lyophilized antibody at 2-8°C for up to 12 months after date of receipt. After reconstitution keep undiluted aliquots at -20°C for up to 6 months for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.

The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF.