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Anti-Connexin-40 (Cx40) Antibody

Our Anti-Connexin-40 (Cx40) sheep polyclonal primary antibody detects rat Connexin-40 (Cx40), and is whole serum. It is validated for use in IHC, WB.

Product Specifications

Background

FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.

Short Description

Our Anti-Connexin-40 (Cx40) sheep polyclonal primary antibody detects rat Connexin-40 (Cx40), and is whole serum. It is validated for use in WB.

Synonyms

Gap junction alpha-5 protein; Cx40; Gja5; Cxn-40

UniProt

P28234

Reactivity

Rat

Immunogen

A synthetic peptide consisting of amino acids 254 to 270 of the C-terminus of rat Cx40 (Cx40/254) conjugated to diphtheria toxoid has been used as the immunogen.

Target

Connexin-40 (Cx40)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IHC, WB

Format

Whole serum

Molecular Weight

40 kDa (See application details.)

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

IHC Dilution

1:100-1:250

WB Dilution

0.7361111111111112

Specificity

It is reported that the Cx40/254 antibody did not cross react with rat Cx43 but may with Cx45 in Western blots only (Rummery, NM et al 2002) .

Host or Source

Sheep

Physical Properties

Lyophilized

Isotype

IgG

Immunogen Species

Rat

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