Anti-Neurotrophin-3 (NT-3) Antibody

Our Anti-Neurotrophin-3 (NT-3) sheep polyclonal primary antibody detects chicken, human, and rat Neurotrophin-3 (NT-3), and is affinity-purified. It is validated for use in ELISA, IHC-Frozen, Neutralize, WB.

Product Specifications

Background

FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.

Short Description

Synonyms

Neurotrophin-3; Neurotrophic factor; HDNF; Nerve growth factor 2; NGF-2; Ntf3; Ntf-3

UniProt

P20783

Reactivity

Chicken, Human, and Rat

Immunogen

A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.

Target

Neurotrophin-3 (NT-3)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, IHC-Frozen, Neutralize, WB

Format

Affinity-purified

Buffer

Lyophilized from a solution containing 3% Trehalose in PBS pH 7.4

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute in 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Notes

Affinity purified antibody

IHC Dilution

1-10 μg/mL

WB Dilution

1-10 μg/mL

Specificity

A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.