Anti-Beta-nerve growth factor (beta-NGF) Antibody

Our Anti-Beta-nerve growth factor (beta NGF) sheep polyclonal primary antibody detects avian, human, mouse, and rat Beta-nerve growth factor (beta NGF), and is affinity-purified. It is validated for use in ELISA, IHC-Frozen, Neutralize, WB.

Product Specifications

Background

FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.

Short Description

Synonyms

Beta-nerve growth factor

UniProt

P01139

Reactivity

Avian, Human, Mouse, and Rat

Immunogen

Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)

Target

Beta-nerve growth factor (beta NGF)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, IHC-Frozen, Neutralize, WB

Format

Affinity-purified

Buffer

Lyophilized from a solution containing 0.1% Trehalose in PBS pH 7.4

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute in 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Notes

Affinity purified antibody

IHC Dilution

1-3 μg/mL

WB Dilution

1-3 μg/mL

Specificity

A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.