Anti-Laminin-111 Antibody

Our Anti-Laminin-111 rabbit polyclonal primary antibody detects mouse and rat Laminin-111 , and is affinity purified. It is validated for use in IHC-Frozen, WB.

Product Specifications

Background

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ref: uniprot.org

Short Description

Our Anti-Laminin-111 rabbit polyclonal primary antibody detects mouse and rat Laminin-111, and is affinity purified. It is validated for use in IHC-Frozen, WB.

Synonyms

Laminin 1, Laminin _1_1_1.

UniProt

P19137; P02469; P02468

Reactivity

Mouse and Rat

Immunogen

Laminin-111 isolated from mouse EHS cells

Target

Laminin-111

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IHC-Frozen, WB

Format

Affinity purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

IHC Dilution

1:1000-1:5000

WB Dilution

1:1000-1:5000

Specificity

Reacts with mouse and rat, other species not tested. This antibody recognizes laminin isotypes alpha-1 (440 kDa), beta-1 (220 kDa) and gamma-1 (220 kDa) . It also binds laminin binding protein at 120 kDa which always co-expresses with laminin.