Anti-GAPDH Antibody

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), and is whole serum. It is validated for use in ICC, WB.

Product Specifications

Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

Short Description

Synonyms

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH

UniProt

P04406

Reactivity

Human, Mouse, Other Mammals and (Predicted)

Immunogen

Full length recombinant human GAPDH expressed in and purified from E. coli

Target

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, WB

Format

Whole serum

Buffer

Lyophilized with sodium azide.

Molecular Weight

~36-38 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.