Anti-Vimentin Antibody
Our Anti-Vimentin rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Vimentin , and is whole serum. It is validated for use in ICC, WB.
Product Specifications
Background
Vimentin is the major protein subunit of the 10 nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells. Vimentin is also found in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons) . Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.
Short Description
Our Anti-Vimentin rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Vimentin, and is whole serum. It is validated for use in ICC, WB.
UniProt
P08670
Reactivity
Human, Mouse, Other Mammals and (Predicted)
Immunogen
Recombinant human Vimentin purified from E. coli.
Target
Vimentin
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ICC, WB
Format
Whole serum
Buffer
Lyophilized with sodium azide.
Molecular Weight
55 kDa
Precautions
For research use only.
Shipping Conditions
25°C (ambient)
Storage Conditions
Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
WB Dilution
1:5000-1:10000
Specificity
Specific for the ~55 kDa Vimentin protein.
ICC Dilution
1:500-1:1000
Host or Source
Rabbit
Physical Properties
Lyophilized
Isotype
IgG
Immunogen Species
Human
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items