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Anti-Vimentin Antibody

Our Anti-Vimentin rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Vimentin , and is whole serum. It is validated for use in ICC, WB.

Product Specifications

Background

Vimentin is the major protein subunit of the 10 nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells. Vimentin is also found in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons) . Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.

Short Description

Our Anti-Vimentin rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Vimentin, and is whole serum. It is validated for use in ICC, WB.

UniProt

P08670

Reactivity

Human, Mouse, Other Mammals and (Predicted)

Immunogen

Recombinant human Vimentin purified from E. coli.

Target

Vimentin

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, WB

Format

Whole serum

Buffer

Lyophilized with sodium azide.

Molecular Weight

55 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

WB Dilution

1:5000-1:10000

Specificity

Specific for the ~55 kDa Vimentin protein.

ICC Dilution

1:500-1:1000

Host or Source

Rabbit

Physical Properties

Lyophilized

Isotype

IgG

Immunogen Species

Human

Curated Selection

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