Anti-Choline O-acetyltransferase (ChAT) Antibody

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

Our Anti-Choline O-acetyltransferase (ChAT) rabbit polyclonal primary antibody detects guinea pig, human, pig, rabbit, and rat Choline O-acetyltransferase (ChAT), and is affinity-purified. It is validated for use in ELISA, IHC-Frozen.

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

This antibody stains cholinergic neurons in human, rat, guinea-pig and rabbit central and peripheral nervous systems. This antiserum is known to react with ChAT of origin: human, rat, guinea-pig and rabbit.