Anti-Autophagy-related protein 3 (ATG3) Antibody

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3) -modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme) . Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.

Our Anti-Autophagy-related protein 3 (ATG3) rabbit polyclonal primary antibody detects human, mouse, and rat Autophagy-related protein 3 (ATG3), and is whole serum. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, WB.

A synthetic peptide corresponding to a central region of the human ATG3 protein [aa. 151 EGEAADMEEYE 161] conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is highly conserved and homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey), Canis familiaris (dog), and many more species.

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

IHC, WB and ELISA confirmed the specificity for ATG3. A 37 kDa band, that corresponds to the molecular weight of ATG3, is detected via Western blot analysis. Human, rat, mouse. Other species not yet tested.