Anti-MAP1LC3B Antibody

MAP1LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B is probably involved in formation of autophagosomal vacuoles (autophagosomes) . SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.

Our Anti-Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B) rabbit polyclonal primary antibody detects human and rat Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B), and is whole serum. It is validated for use in IHC-Frozen, WB.

Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated protein 1 light chain 3 beta; MAP1A/MAP1B LC3 B; MAP1A/1B light chain 3 B; MAP1 light chain 3-like protein 2; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; APG8b; MAP-1LC3B; MAP1LC3B; MAP1ALC3

A synthetic peptide (FEQRVEDVRLIC) corresponding to the N-terminal of human MAP1LC3 B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B protein.

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.