Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Antibody

Our Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) rabbit polyclonal primary antibody detects bovine, chicken, horse, human, mouse, pig, and rat Ubiquitin C Terminal Hydrolase 1 (UCHL1), and is whole serum. It is validated for use in ICC, WB.

Product Specifications

Background

This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT) .

Short Description

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1

UniProt

P09936

Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, and Rat

Immunogen

Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.

Target

Ubiquitin C Terminal Hydrolase 1 (UCHL1)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, WB

Format

Whole serum

Buffer

Lyophilized with sodium azide.

Molecular Weight

24 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

WB Dilution

1:1000-1:2000

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, rat, Ms, Bov, Por.