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Anti-Neuron specific enolase (NSE) Antibody

Our Anti-Neuron specific enolase (NSE) rabbit polyclonal primary antibody detects human, mouse, and rat Neuron specific enolase (NSE), and is whole serum. It is validated for use in ICC, WB.

Product Specifications

Background

Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase) . These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.

Short Description

Our Anti-Neuron specific enolase (NSE) rabbit polyclonal primary antibody detects human, mouse, and rat Neuron specific enolase (NSE), and is whole serum. It is validated for use in ICC, WB.

Synonyms

Gamma-enolase; EC 4.2.1.11; 2-phospho-D-glycerate hydrolyase; Neural enolase; Neuron-specific enolase; NSE; Enolase 2; ENO2

UniProt

P09104

Reactivity

Human, Mouse, and Rat

Immunogen

Recombinant human Neuron Specific Enolase (NSE) expressed in and purified from E.coli

Target

Neuron specific enolase (NSE)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, WB

Format

Whole serum

Buffer

Lyophilized with sodium azide.

Molecular Weight

47 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

IHC Dilution

0.3888888888888889

WB Dilution

1:1000-1:2000

Specificity

Specifically recognizes ~47 kDa NSE protein in WB. Human and rat. Predicted to react with other mammals due to sequence homology.

Host or Source

Rabbit

Physical Properties

Lyophilized

Isotype

Mixed

Immunogen Species

Human

Curated Selection

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