Anti-Neurofilament light polypeptide (NF-L) Antibody
Our Anti-Neurofilament light polypeptide (NF-L) rabbit polyclonal primary antibody detects bovine, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is whole serum. It is validated for use in IF, ICC, IHC, WB.
Product Specifications
Background
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Short Description
Our Anti-Neurofilament light polypeptide (NF-L) rabbit polyclonal primary antibody detects bovine, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is whole serum. It is validated for use in IF, ICC, IHC, WB.
Synonyms
NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL; neurofilament light chain; Neurofilament triplet L protein; NFL
UniProt
P07196
Reactivity
Bovine, Human, Mouse, Pig, and Rat
Immunogen
The antibody has been made against a preparation of recombinant full length human NF-L. The antibody binds NF-L from a variety of species including human, rat and mouse.
Target
Neurofilament light polypeptide (NF-L)
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
IF, ICC, IHC, WB
Format
Whole serum
Buffer
Lyophilized with sodium azide.
Precautions
For research use only.
Shipping Conditions
25°C (ambient)
Storage Conditions
Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
IHC Dilution
3.513888888888889
WB Dilution
1:10000-1:15000
Specificity
Species cross-reactivity includes human, rat, mouse, cow, and pig.
ICC Dilution
1:2000-1:5000
Host or Source
Rabbit
Physical Properties
Lyophilized
Isotype
Mixed
Immunogen Species
Human
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