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Anti-His-Tag Antibody

Our Anti-His-Tag rabbit polyclonal primary antibody detects species independent His-Tag , and is affinity-purified. It is validated for use in ELISA, ICC, IHC-Frozen, WB.

Product Specifications

Background

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Short Description

Our Anti-His-Tag rabbit polyclonal primary antibody detects His-Tag, and is affinity-purified. It is validated for use in ELISA, ICC, IHC-Frozen, WB.

Reactivity

Species Independent

Immunogen

The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.

Target

His-Tag

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ELISA, ICC, IHC-Frozen, WB

Format

Affinity-purified

Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Precautions

For research use only.

Shipping Conditions

2-8°C (on cold packs)

Storage Conditions

Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.

IHC Dilution

5 μg/mL

WB Dilution

1:500-1:1000

IP Dilution

5 μg/sample

Specificity

His-tagged fusion proteins.

ICC Dilution

5 μg/mL

Host or Source

Rabbit

Physical Properties

Liquid

Isotype

IgG

Immunogen Species

Species Independent

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