Anti-D Tag Antibody

Our Anti-D Tag rabbit polyclonal primary antibody detects species independent D Tag , and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.

Product Specifications

Background

The D-tag system utilises a short hydrophilic peptide (DYKDDDDK) that is fused to either the N- or C-terminus of the protein of interest. It can be used in conjunction with other tags such as the 6X His tag.

Short Description

Our Anti-D Tag rabbit polyclonal primary antibody detects D Tag, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.

Reactivity

Species Independent

Immunogen

A synthetic peptide (DYKDDDDK) coupled to KLH.

Target

D Tag

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, IHC-Frozen, WB

Format

Affinity-purified

Buffer

PBS, pH 7.4 with 0.05% sodium azide

Precautions

For research use only.

Shipping Conditions

2-8°C (on cold packs)

Storage Conditions

Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Affinity chromatography using epitope-specific peptide.

IHC Dilution

0.18055555555555555

WB Dilution

0.7361111111111112

IP Dilution

0.18055555555555555

Specificity

Anti D-tag antibody recognizes over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.