Anti-D Tag Antibody
Our Anti-D Tag rabbit polyclonal primary antibody detects species independent D Tag , and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.
Product Specifications
Background
The D-tag system utilises a short hydrophilic peptide (DYKDDDDK) that is fused to either the N- or C-terminus of the protein of interest. It can be used in conjunction with other tags such as the 6X His tag.
Short Description
Our Anti-D Tag rabbit polyclonal primary antibody detects D Tag, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.
Reactivity
Species Independent
Immunogen
A synthetic peptide (DYKDDDDK) coupled to KLH.
Target
D Tag
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
ICC, IHC-Frozen, WB
Format
Affinity-purified
Buffer
PBS, pH 7.4 with 0.05% sodium azide
Precautions
For research use only.
Shipping Conditions
2-8°C (on cold packs)
Storage Conditions
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Affinity chromatography using epitope-specific peptide.
IHC Dilution
0.18055555555555555
WB Dilution
0.7361111111111112
IP Dilution
0.18055555555555555
Specificity
Anti D-tag antibody recognizes over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
ICC Dilution
0.18055555555555555
Host or Source
Rabbit
Physical Properties
Liquid
Isotype
IgG
Immunogen Species
Species Independent
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