Anti-Orexin-A Antibody

Our Anti-Orexin-A rabbit polyclonal primary antibody detects bovine, and rat Orexin-A , and is whole serum. It is validated for use in IHC-Frozen.

Product Specifications

Background

FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.

Short Description

Our Anti-Orexin-A rabbit polyclonal primary antibody detects bovine, and rat Orexin-A, and is whole serum. It is validated for use in IHC-Frozen.

Synonyms

Orexin-A; Hypocretin-1; Hcrt1

UniProt

P56717

Reactivity

Bovine, and Rat

Immunogen

A synthetic peptide (CRLYELLHGAGNHAAGILTL) as part of Bovine Orexin A (aa: 14-33) conjugated to KLH has been used as the immunogen.

Target

Orexin-A

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IHC-Frozen

Format

Whole serum

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

IHC Dilution

1:1000-1:2000

Specificity

The specificity for this antiserum has been confirmed by immunohistochemistry on rat brain and the results reflect the current literature. This antibody is known to react with rat Orexin A.