Anti-Neuronal nuclei antigen (NeuN/FOX3) Antibody (1B7)

FOX3 is one of a family of mammalian homologues of FOX1 (FOX is an acronym of "Feminizing locus on X") . The FOX proteins are about 46 kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on FOX3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/FOX3 functions as a Pre-mRNA alternative splicing regulator. It regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD) and is expressed heavily and specifically in neuronal nuclei and cytoplasm. In the cerebellum, Fox3 does not stain Purkinje neurons or Golgi neurons. The antibody was raised against the N-terminal (100 amino acids) of human FOX3, as expressed in and purified from E. coli. The full length FOX3 is not used as the immunogen since the three mammalian FOX homologues, namely FOX1, FOX2 and FOX3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules, so antibodies specific for each of the three molecules can therefore be generated.

Our Anti-Neuronal nuclei antigen [NeuN] / RNA binding protein fox-1 homolog 3 [FOX3] (NeuN/FOX3) mouse monoclonal primary antibody detects human, mouse, and rat Neuronal nuclei antigen [NeuN] / RNA binding protein fox-1 homolog 3 [FOX3] (NeuN/FOX3), and is IgG. It is validated for use in IF, ICC, IHC, WB.

The antibody has been made against a recombinant construct, based on the N-terminal (100 amino acids) of human FOX3 expressed in and purified from E Coli.

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.