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Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Selected Variant

M-1827-100

Size: 100 µg

Our Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Glial Fibrillary Acidic Protein (GFAP), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Product Specifications

Background

GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.

Short Description

Our Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Glial Fibrillary Acidic Protein (GFAP), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Synonyms

Glial fibrillary acidic protein; GFAP

UniProt

Q864W9

Reactivity

Bovine, Human, Mouse, Pig, and Rat

Immunogen

GFAP isolated biochemically from pig spinal cord was used as the immunogen.

Target

Glial Fibrillary Acidic Protein (GFAP)

Clonality

Monoclonal

Clone

2A5

Conjugation

Unconjugated

Applications

IHC-Frozen, WB

Format

Affinity-purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Molecular Weight

50 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Notes

Protein G purified

IHC Dilution

1:500-1:1000

WB Dilution

1:1000-1:2000

Specificity

This antibody is specific for GFAP as demonstrated by Western blotting and immunohistochemistry.

Host or Source

Mouse

Physical Properties

Lyophilized

Isotype

IgG1

Immunogen Species

Pig

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