Anti-Amyloid beta peptide (A-beta 40/42) Antibody (Biotin)

Our Anti-Amyloid beta peptide (A-beta 40/42) mouse monoclonal primary antibody detects human and rat Amyloid beta peptide (A-beta 40/42), and is igg. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB.

Product Specifications

Background

MOAB-2 did not detect APP or APP-CTFsbiotinylated MOAB-2antibodyhere

Short Description

Our Anti-Amyloid beta peptide (A-beta 40/42) mouse monoclonal primary antibody detects human and rat Amyloid beta peptide (A-beta 40/42), and is IgG. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB.

Synonyms

Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein; MOAB2; MOAB-2; Alzheimer's antibody; AB40; AB42; abeta

UniProt

P05067

Reactivity

Human and Rat

Immunogen

Recombinant human amyloid beta protein 42 (Aβ42) : DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

Target

Amyloid beta peptide (A-beta 40/42)

Clonality

Monoclonal

Clone

MOAB-2

Conjugation

Unconjugated

Applications

ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded, IP, WB

Assay Protocol

MOAB-2_extended product_information_M-1586-100_M-1752-50_2024.pdf MOAB-2_extended product_information_M-1586-100_M-1752-50_2024.pdf

Format

IgG

Buffer

Lyophilized from PBS buffer, pH 7.4; contains no preservative.

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute in 50 μL sterile-filtered, ultrapure water to give a concentration of 1 mg/mL. Centrifuge to remove any insoluble material. Final buffer is PBS, pH 7.4 without preservative.After reconstitution keep aliquots at -20°C to -70°C for a higher stability. At 2-8°C keep up to one week; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.

Notes

Antibody was purified from cell culture supernatant by Protein G chromatography, biotinylated and buffer-exchanged into PBS, pH 7.4 buffer

IHC Dilution

1:500-1:2000

WB Dilution

1:2000-1:5000

IP Dilution

1:200-1:1000

Specificity

MOAB-2 detects preparations enriched in U-, O-, F-Aβ42, and U-Aβ40 by dot-blot, and is thus a pan-specific Aβ antibody. However, MOAB-2 is selective for the more neurotoxic Aβ42 compared to Aβ40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects Aβ40 at 2.5 pmol, but U-, O- and F-Aβb42 at antigen concentrations as low as ~ 0.1 pmol (Youmans. KL et al., 2012; PMID: 22423893) . MOAB-2 does not detect APP (Amyloid Precursor Protein) . Human, rat, other species not yet tested. By Dot Blot, MOAB-2 detected rat Aβ40 and human Aβ40, albeit with less affinity than for Aβ42 (Youmans KL et al., 2012) .