Anti-Capsaicin receptor (VR1/TrpV1) Antibody

The capsaicin receptor (VR1, TRPV1) is a ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. The receptor seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. It is involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH less than 6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (Ref: uniprot.org) .

Our Anti-Capsaicin receptor (TrpV1) mouse monoclonal primary antibody detects guinea pig (predicted), mouse, and rat Capsaicin receptor (TrpV1), and is IgG. It is validated for use in FC, WB.

VR1; Transient receptor potential cation channel subfamily V member 1; TrpV1; osm-9-like TRP channel 1; OTRPC1; Vanilloid receptor 1; Capsaicin receptor; VR-1

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives but will contain 0.1% trehalose and buffer salts. Store lyophilized antibody at 2-8°C. After reconstitution divide in to aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Storage at 2-8°C with an appropriate antibacterial agent. USE Sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.

Antibody is specific for rat/mouse VR1 protein in Westerns and immunofluorescent immunohistochemistry on mouse PEG fixed DRG tissues. Pre-absorption with immunogen obliterates positive staining. Cross reactivity with other non-VR1 proteins is minimal; cross reactivity with VR1 from other species not yet tested. This antibody clone is known to react with rat and mouse TrpV1. It is predicted to react with guinea pig due to sequence homology.