Anti-Ataxin-2 binding protein 1 (A2BP1/Fox1) Antibody

Fox1 is one of a family of 3 mammalian Fox1 homologues. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X" (1) . The C. elegans Fox1 protein and its 3 mammalian homologues are all about 46 kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2) . As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3) . Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5) . Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6) .

Our Anti-Ataxin-2 binding protein 1 (A2BP1) mouse monoclonal primary antibody detects human, mouse, and rat Ataxin-2 binding protein 1 (A2BP1), and is IgG. It is validated for use in IHC-Frozen, WB.

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

The antibody reacts with a 48 kDa and 46 kDa band by Western blot on extract from mouse brain. It has also been used successfully for immunocytochemistry on rat neuronal cultures.