Anti-Microtubule Associated Protein 2 (MAP2) Antibody

Microtubules are 25 nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one 'a' subunit and one 'b' tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2 a and MAP2 b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau) . MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.

Our Anti-Microtubule Associated Protein 2 (MAP2) mouse monoclonal primary antibody detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2), and is IgG. It is validated for use in ICC, IHC-Frozen, WB.

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations) . After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.