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Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) Antibody

Our Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), and is igg. It is validated for use in ICC, IHC-Frozen, WB.

Product Specifications

Background

This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT) .

Short Description

Our Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), and is IgG. It is validated for use in ICC, IHC-Frozen, WB.

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1

UniProt

P09936

Reactivity

Bovine, Human, Mouse, Pig, and Rat

Immunogen

Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.

Target

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1)

Clonality

Monoclonal

Clone

BH7

Conjugation

Unconjugated

Applications

ICC, IHC-Frozen, WB

Format

IgG

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Molecular Weight

24 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Notes

Protein G purified

IHC Dilution

1:1000-1:5000

WB Dilution

1:10000-1:20000

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord, brain, SHSY-5Y or HEK293 cell extract. Hu, rat, Bov.

ICC Dilution

1:1000-1:5000

Host or Source

Mouse

Physical Properties

Lyophilized

Isotype

IgG1

Immunogen Species

Human

Frequently Asked Questions

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