Anti-Alpha-Synuclein Antibody

Our Anti-Alpha-synuclein mouse monoclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Alpha-synuclein , and is igg. It is validated for use in ICC, WB.

Product Specifications

Background

Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies) . Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.

Short Description

Our Anti-Alpha-synuclein mouse monoclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Alpha-synuclein, and is IgG. It is validated for use in ICC, WB.

Synonyms

Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

UniProt

P37840

Reactivity

Bovine, Chicken, Horse, Human, Mouse, Other Mammals (Predicted), Pig, and Rat

Immunogen

Recombinant full length human alpha synuclein expressed and purified from E. coli

Target

Alpha-synuclein

Clonality

Monoclonal

Clone

3H9

Conjugation

Unconjugated

Applications

ICC, WB

Format

IgG

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Molecular Weight

14-15 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, divide into single use aliquots and store at -20-80°C for a higher stability. Avoid freeze-thaw cycles.

Notes

Protein G purified

IHC Dilution

1:500-1:3000

WB Dilution

1:1000-1:5000

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~14-15 kDa alpha synuclein protein. The epitope for 3H9 is in the region 61-95 which correspond to the Non-Amyloid beta Component of Alzheimer's disease amyloid (NAC) region. 3H9 will also bind human alpha-synuclein containing the A30P and A53T mutations. Human, horse, cow, pig, chicken, rat, mouse. Predicted to react with other mammalian tissue because of highly conserved nature of the protein.