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Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) mouse monoclonal primary antibody detects bovine, chicken, human, mouse, pig, and rat Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), and is purified. It is validated for use in ICC, IHC-Frozen, WB.

Product Specifications

Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

Short Description

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) mouse monoclonal primary antibody detects bovine, chicken, human, mouse, pig, and rat Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), and is purified. It is validated for use in ICC, IHC-Frozen, WB.

Synonyms

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH

UniProt

P00355

Reactivity

Bovine, Chicken, Human, Mouse, Pig, and Rat

Immunogen

Purified pig GAPDH

Target

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Clonality

Monoclonal

Clone

1D4

Conjugation

Unconjugated

Applications

ICC, IHC-Frozen, WB

Format

Purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Molecular Weight

36 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 250 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Notes

Protein L purified

WB Dilution

0.7361111111111112

Specificity

The specificity of this antibody has been confirmed by WB. Human, rat, mouse, bovine, Porcine, Chicken.

ICC Dilution

0.1111111111111111

Host or Source

Mouse

Physical Properties

Lyophilized

Isotype

IgM

Immunogen Species

Pig

Curated Selection

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