Anti-Fibrillarin (Nop1p) Antibody

Fibrillarin is part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin such as this antibody can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.

Our Anti-rRNA 2'O-methyltransferase fibrillarin (Nop1p) mouse monoclonal primary antibody detects C. elegans, drosophila, human, other mammals (predicted), rat, and yeast rRNA 2'O-methyltransferase fibrillarin (Nop1p), and is IgG. It is validated for use in ICC, WB.

Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae) .

Spin the vial briefly before opening it. Reconstitute in 250 uL sterile-filtered, room temperature, ultrapure water. Let the vial rehydrate for 5 minutes. Mix gently with a pipette tip to help the redissolution of the material. Centrifuge to remove any insoluble material. The final solution will contain no preservatives. A sterile technique is recommended.After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on Western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.