Anti-Ki-67 Antibody

Our Anti-Ki-67 chicken polyclonal primary antibody detects human Ki-67, and is igy preparation. It is validated for use in ICC, WB.

Product Specifications

Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226) . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226) . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226) . Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551) . Does not contribute to the internal structure of mitotic chromosomes (By similarity) . May play a role in chromatin organization (PubMed:24867636) . It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable) . Ref: uniprot.org

Short Description

Our Anti-Ki-67 chicken polyclonal primary antibody detects human Ki-67, and is IgY preparation. It is validated for use in ICC, WB.

Synonyms

Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67

UniProt

P46013

Reactivity

Human

Immunogen

E. coli.

Target

Ki-67

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, WB

Format

IgY preparation

Buffer

Lyophilized IgY preparation, with sodium azide.

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.