Anti-Vimentin Antibody

Our Anti-Vimentin chicken polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Vimentin, and is igy preparation. It is validated for use in ICC, IHC-Frozen, WB.

Product Specifications

Background

Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas.

Short Description

Our Anti-Vimentin chicken polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Vimentin, and is IgY preparation. It is validated for use in ICC, IHC-Frozen, WB.

Synonyms

VIM

UniProt

P08670

Reactivity

Human, Mouse, Other Mammals (Predicted), and Rat

Immunogen

Recombinant human Vimentin purified from E.coli

Target

Vimentin

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, IHC-Frozen, WB

Format

IgY preparation

Buffer

Lyophilized IgY preparation, with sodium azide.

Molecular Weight

50 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

WB Dilution

1:5000-1:10000

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~50 kDa Vimentin enzyme. Hu, rat, Ms. It is predicted to react with other mammals due to sequence homology.