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Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) Antibody

Our Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) chicken polyclonal primary antibody detects bovine, human, mouse, other mammals (predicted), pig, and rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5), and is igy preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Product Specifications

Background

This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT) .

Short Description

Our Anti-Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5) chicken polyclonal primary antibody detects bovine, human, mouse, other mammals (predicted), pig, and rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Synonyms

Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1; Ubiquitin C Terminal Hydrolase 1

UniProt

P09936

Reactivity

Bovine, Human, Mouse, Other Mammals (Predicted), Pig, and Rat

Immunogen

Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.

Target

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (PGP9.5)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

ICC, IHC-Frozen, IHC-Paraffin-embedded, WB

Format

IgY preparation

Buffer

Lyophilized IgY preparation, with sodium azide.

Molecular Weight

24 kDa

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

WB Dilution

1:5000-1:10000

Specificity

The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, rat, Ms, Bov, Por. Predicted to react with other mammalian tissues due to sequence homology.

ICC Dilution

1:500-1:1000

Host or Source

Chicken

Physical Properties

Lyophilized

Isotype

IgY

Immunogen Species

Human

Frequently Asked Questions

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