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Anti-Neurofilament light polypeptide (NF-L) Antibody

Our Anti-Neurofilament light polypeptide (NF-L) chicken polyclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is igy preparation. It is validated for use in IF, ICC, IHC, WB.

Product Specifications

Background

Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.

Short Description

Our Anti-Neurofilament light polypeptide (NF-L) chicken polyclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgY preparation. It is validated for use in IF, ICC, IHC, WB.

Synonyms

NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL; neurofilament light chain; Neurofilament triplet L protein; NFL

UniProt

P07196

Reactivity

Bovine, Horse, Human, Mouse, Pig, and Rat

Immunogen

The antibody has been made against a preparation of recombinant full length human NF-L. The antibody binds NF-L from a variety of species including human, rat and mouse.

Target

Neurofilament light polypeptide (NF-L)

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

IF, ICC, IHC, WB

Format

IgY preparation

Buffer

Lyophilized IgY preparation, with sodium azide.

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

IHC Dilution

1:1000-1:5000

WB Dilution

1:5000-1:10000

Specificity

Species cross-reactivity includes human, rat, mouse, cow and pig. Predicted to react with other mammalian tissues due to sequence homology.

ICC Dilution

1:1000-1:5000

Host or Source

Chicken

Physical Properties

Lyophilized

Isotype

IgY

Immunogen Species

Human

Frequently Asked Questions

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