Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70 kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)

Our Anti-Neurofilament heavy polypeptide, phosphorylated (pNF-H) chicken polyclonal primary antibody detects bovine, dog, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament heavy polypeptide, phosphorylated (pNF-H), and is IgY preparation. It is validated for use in ELISA, IF, ICC, IHC, WB.

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

Species cross-reactivity includes human, rat, mouse, cow, pig, horse and dog. This antibody reacts with phosphorylated NF-H and is seen as a band at approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. Predicted to react with other mammalian tissues due to sequence homology.