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Bulk Human IgG4 (IB4) Isotype Control S228P - Extremely Low Endotoxin

Product Specifications

Background

Recombinant IgG4 human antibody

Host

CHO

Reactivity

Human

Immunogen

Hen Egg Lysozyme (HEL)

Target

IgG4

Clone

IB4

Applications

Flow Cytometry, Functional Assays

Purification Method

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Concentration

5mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Fragment

Human IgG4 S228P Kappa

Specificity

This Human IgG4 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG4 antibody is suitable for in vivo applications. This Human IgG4 isotype control contains the S228P mutation

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

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