Bulk Anti-DYKDDDDK Tag (M2-ICH) Antibody - Human IgG1

Product Specifications

Background

Ichorbio's in vivo grade recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone M2-ICH) is a chimeric monoclonal antibody produced in mammalian cells.

Product Name Alternative

FLAG tag, M2 clone, DYKDDDDK peptide

Host

Human

Immunogen

DYKDDDDK tag

Target

DYKDDDDK-tag

Isotype

IgG1

Clone

Recombinant version of the M2 clone

Applications

ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.

Purification Method

The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.

Concentration

1mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This low endotoxin FLAG-tag antibody is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Alternative of the monoclonal FLAG M2 antibody for the detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only.