Bulk Anti-DYKDDDDK Tag (M2-ICH) Antibody - Mouse IgG2a
Product Specifications
Background
Ichorbio's in vivo grade recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone M2-ICH) is a chimeric monoclonal antibody produced in mammalian cells.
Product Name Alternative
FLAG tag, M2 clone, DYKDDDDK peptide
Host
Rat
Immunogen
DYKDDDDK tag
Target
DYKDDDDK-tag
Isotype
IgG2a
Clone
Recombinant version of the M2 clone
Applications
ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls.
Purification Method
This monoclonal antibody was purified using Protein A
Concentration
1mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Shipping Conditions
Blue ice
Storage Conditions
This low endotoxin FLAG-tag antibody is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Specificity
Alternative of the monoclonal FLAG M2 antibody for the detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Isotype Control
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
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