Anti-Mouse CD317 (927) In Vivo Antibody - Low Endotoxin

Product Specifications

Host

Rat

Reactivity

Mouse

Immunogen

Mouse plasmacytoid dendritic cells

Target

CD317

Isotype

IgG2a κ

Clone

927

Applications

Depletion, Functional Assays, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Clone 927 activity is directed against murine CD317 (BST2; PDCA-1) .

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly (I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation.