Anti-Mouse MHC Class I H-2Kb (AF6-88.5) In Vivo Antibody - Low Endotoxin

Product Specifications

Host

Mouse

Reactivity

Mouse

Immunogen

Mouse C57BL Splenocytes

Target

MHC Class I

Isotype

IgG2a κ

Clone

AF6-88.5

Applications

Functional Assays, Flow Cytometry, IHC Frozen, Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Clone AF6-88.5, Anti-Mouse H-2Kb, recognizes an epitope on mouse MHC class I H-2K haplotype b. AF6-88.5 does not cross-react with other H-2K haplotypes, including d, f, j, k, p, q, r, s, u, v.

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen and is ubiquitously expressed on nucleated cells from mice of the H-2Kb haplotype, including C57BL/6. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins