Anti-Mouse Ly49C (4LO3311) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.

Product Name Alternative

Ly-49 inhibitory receptors C

Host

Mouse

Reactivity

Mouse

Immunogen

4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells

Target

Ly49C

Isotype

IgG3 κ

Clone

4LO3311

Applications

Depletion, Flow Cytometry, Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

4LO3311 activity is directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1) . 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain.