Anti-Mouse CD183 (CXCR3-173) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Hamsters were tested by ELISA for seropositivity against CXCR3 peptide and hybridomas were generated, screened by FACS, purified, and tested for: staining, CXCR3 blockade in vitro, and endotoxin levels. CXCR3-173 detects the native form of CXCR3, and therefore does not work in Western blotting. CXCR3-173 has potential use in immunotherapeutic approaches to inhibit transplant rejection and immune related diseases. In vitro, CXCR3-173 blocks chemotaxis in response to CXCL10 or CXCL11 but not CXCL9. In vivo, CXCR3-173 prolongs both cardiac and islet allograft survival in a manner further enhanced by rapamycin.

Product Name Alternative

CXCR3G, protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R), CD183

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

CXCR3-173 was generated by immunizing Armenian hamsters with a peptide sequence unique to mouse CXCR3 which encompasses amino acids 1-37.

Target

CD183

Isotype

IgG

Clone

CXCR3-173

Applications

Flow Cytometry, Neutralization

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

CXCR3-173 activity is directed against murine CD183 (CXCR3) .

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

CXCL3 is expressed on primary memory phenotype CD4+ and CD8+ T cells, naturally occurring CD4+CD25+ Foxp3+ regulatory T cells, natural killer (NK) T cells, and approximately 25% of NK cells. CXCR3-173 recognizes an epitope of CXCR3 expressed on the surface of activated mouse splenocytes.