Bulk anti-Human CXCR4 (12G5) Antibody

Product Specifications

Background

12G5 binds specifically to both human and nonhuman cells that express recombinant CXCR4. 12G5 inhibits CD4-independent infection by some HIV-2 isolates, and preincubating cells with 12G5 abolishes syncytium formation. HIV-2/vcp-infected cells display a marked and selective reduction in 12G5 binding. 12G5 also inhibits induction of cell-to-cell fusion of CXCR4+ RD/CD4 cells by HIV-1 and HIV-2 strains.

Product Name Alternative

Fusin, LESTR, CD184

Host

Mouse

Reactivity

Human

Immunogen

12G5 was produced by immunizing Balb/c mice with CP-MAC-infected Sup-T1 cells. Hybridomas were generated and screened for the ability to inhibit CP-MAC-induced syncytium induction on Sup-T1 cells.

Target

CXCR4

Isotype

IgG2a κ

Clone

12G5

Applications

Blocking, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy, Immunohistochemistry, Neutralization, Western Blotting

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

12G5 activity is directed against human CXCR4 (CD184; Fusin)

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

CXCR4 is expressed in various organs including ovary, bone marrow, kidney, lung, small intestine, spleen, lymph nodes, brain, stomach, liver, thymus, heart, and pancreas as well as on the surface of endothelial mature and precursor cells and pericytes.