Anti-Mouse CD24 (M1/69) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Mouse CD24 is a small 27 amino acid sialoglycoprotein that is anchored to plasma membranes via a glycosyl-phosphatidylinositol linker. CD24 is widely distributed and plays a role in many diverse functions including adaptive immunity, inflammation, autoimmunity, and cancer. CD24 modulates growth and differentiation signals to granulocytes and B cells, is required for homeostatic cell renewal, binds to P-selectin on activated endothelial cells, plays a role in cell adhesion, lymphocyte proliferation for homeostatic purposes4, lymphocyte costimulation via CD28-independent pathways as well as a crucial role in cell selection and maturation during hematopoiesis.

Product Name Alternative

Heat Stable Antigen, Nectadrin, Ly-52

Host

Rat

Reactivity

Mouse

Immunogen

M1/69 was generated by fusing the spleen cells of a DA rat immunized with B10 mouse spleen cells enriched for T cells with cells from a nonsecreting mouse myeloma line (NSI)

Target

CD24

Isotype

IgG2b κ

Clone

M1/69

Applications

Inhibitor, ELISA Indirect, Flow Cytometry, Immunofluorescence, IF Microscopy, Immunohistochemistry, Immunoprecipitation, Western Blot

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-CD24 antibody (clone M1/69) activity is directed against mouse CD24, also known as Heat Stable Antigen (HSA) or Ly-52.

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

CD24 is expressed on B cells, T cells, neutrophils, eosinophils, macrophages, neural cells, ganglion cells, keratinocytes, muscle cells, pancreas cells, lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells and is overexpressed in many cancers. Expression varies during T and B cell differentiation. Peripheral T cells are mainly negative.