Bulk Anti-Human CD166 (3A6) Antibody

Product Specifications

Background

3A6 was produced by immunizing mice with human thymic epithelial cells and then fusing spleen cells with P3X63Ag8 myeloma cells. 3A6 cross reacts with ovine mesenchymal stromal cells from iliac crest bone marrow aspirates

Product Name Alternative

CD6 ligand, Activated Leukocyte Cell Adhesion Molecule, ALCAM

Host

Mouse

Reactivity

Human

Immunogen

This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.

Target

CD166

Isotype

IgG1 κ

Clone

3A6

Applications

Flow Cytometry, Immunofluorescence, IF Microscopy, Immunohistochemistry, Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 2.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

3A6 activity is directed against human CD166 (ALCAM) and cross-reacts with ovine tissues.

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

CD166 is expressed on neurons, activated leukocytes, hematopoietic stem cells, mesenchymal stem cells, bone marrow stromal cells, activated T cells, activated B cells, activated monocytes, thymic epithelial cells, vascular endothelial cells, fibroblasts, keratinocytes, myeloid progenitors, tumor cells, and cancer stem cells.