Anti-Mouse Galectin-9 (RG9-1) In Vivo Antibody - Low Endotoxin

Product Specifications

Background

Galectin-9 (Gal-9), a member of the galectin family of mammalian lectins, binds β-galactoside. Gal-9 is expressed by many cell types, including endothelial cells, the epithelium of the gastrointestinal tract, T cells, B cells, macrophages, and mast cells. Gal-9 plays a significant role in innate and adaptive immunity and regulates excessive immunity by suppressing interleukin (IL) -17 producing effector T helper cells (Th) 17 and Th1 as well as by augmenting Foxp3+ regulatory T cells (Treg) . In addition, Gal-9 induces monocytic myeloid-derived suppressor cells (MDSCs), granulocytic MDSCs, and plasmacytoid dendritic cell-like macrophages. Gal-9 also suppresses B cell receptor signaling and is regulated by I-branching of N-glycans. Additionally, Gal-9 stimulates the maturation of dendritic cells. Gal-9 is thought to function by binding to specific carbohydrate moieties in receptor molecules expressed on the surface of its target cells, including the T-cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3) 1. Gal-9 is predominantly located in the cytoplasm but can also be secreted. Gal-9 is expressed by a variety of tumor cells, plays a role in tumor immunity, and can suppress tumor metastasis by blocking adhesion to endothelium and extracellular matrices. CD45, which regulates antigen receptor signaling, is a major glycoprotein receptor for Gal-9 on naive B cells.

Product Name Alternative

Gal-9

UniProt

https://www.uniprot.org/uniprot/O08573

Host

Rat

Reactivity

Mouse

Immunogen

Recombinant mouse galectin-9

Target

Galectin-9

Isotype

IgG2b κ

Clone

RG9-1

Applications

Blocking

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC, >95% by SDS Page

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

RG9-1 activity is directed against mouse galectin-9

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Antigen Distribution

Galectin-9 is expressed by many cell types, including T cells, B cells, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, endothelial cells, and epithelial cells