Bulk Anti-Human CD5 (UCHT-2) Antibody

Product Specifications

Background

Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.

Product Name Alternative

Leu-1

UniProt

P06127

Host

Mouse

Reactivity

Human

Target

CD5

Isotype

IgG1

Clone

UCHT-2

Applications

CODEX®, Functional Assays, Flow Cytometry, IHC (Frozen)

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC, >95% by SDS Page

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa) .

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Antigen Distribution

The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.

Aggregation

Aggregation level ≤ 5%

Applications Notes

The suggested concentration for this CD5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.