Bulk Anti-Human CD195 (HEK/1/85a/7a) Antibody (CCR5)

CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR) . CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP) -1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection. CCR5 also contributes to other human diseases, including type I diabetes and cancer.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.