Anti-Mouse NKG2A/C/E (20D5) In Vivo Antibody - Low Endotoxin

Product Specifications

Product Name Alternative

CD159a, CD159c, CD159e, NKG2C, NKG2E

Host

Rat

Reactivity

Mouse

Immunogen

CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94

Target

NKG2A/C/E

Isotype

IgG2a

Clone

20D5

Applications

Flow Cytometry

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

1.0 - 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Clone 20D5 antibody reacts with mouse NKG2A, NKG2C, and NKG2E however, this hybridoma has not been epitope mapped to identify the binding site.

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Antigen Distribution

NK cells and NK T cells from C57BL/6 mouse

Aggregation

Aggregation level ≤ 5%

Applications Notes

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 μl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.