Anti-Mouse CD370 (1F6) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
CLEC9A, also known as DNGR1 is defined as a type II membrane protein with a single extracellular C-type lectin domain1. CLEC9A is involved with adaptive immune response during infection. Additionally, CLEC9A, is being investigated for potential to enhance immune responses produced by vaccines.
Product Name Alternative
CD370, DNGR-1, C-Type Lectin Domain Family 9 Member A
UniProt
Q8BRU4
Host
Rat
Reactivity
Mouse
Immunogen
RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope.
Target
CLEC9A
Isotype
IgG1
Clone
1F6
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
>2.0 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Shipping Conditions
Blue ice
Storage Conditions
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Specificity
Clone 1F6 recognizes an epitope on mouse DNGR1
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
Antigen Distribution
CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
Rat IgG1 Isotype Control for In Vivo - Low Endotoxin [GL113] (ICH2246)
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