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Anti-Mouse TCR Beta (H57-597) Antibody In Vivo Antibody - Low Endotoxin

Product Specifications

Background

TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival.

Product Name Alternative

TCR-β chain, TCR-β, β-TCR

Host

Armenian Hamster

Reactivity

Mouse

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Target

TCR Beta

Isotype

IgG

Clone

H57-597

Applications

Flow Cytometry, Costimulatory, Depletion, Immunoprecipitation, IHC (Frozen), IHC (Paraffin), CODEX

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

≥ 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC, >95% by SDS Page

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells.

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Antigen Distribution

TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells.

Aggregation

Aggregation level ≤ 5%

Applications Notes

The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Isotype Control

Armenian Hamster IgG Isotype Control for In Vivo - Ultra Low Endotoxin [PIP] (ICH2251UL)

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