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Anti-Mouse H-2K (Y-3) In Vivo Antibody - Low Endotoxin

Product Specifications

Product Name Alternative

MHC Class 2, H2-K1, histocompatibility 2, K1, K region

Host

Rat

Reactivity

Mouse

Immunogen

Con A stimulated spleen cells from BALB.B mice

Target

H-2K

Isotype

IgG2b κ

Clone

Y-3

Applications

Western Blot, Functional Assays

Purification Method

This monoclonal antibody was purified using Protein A

Concentration

1.0 - 5.0 mg/mL

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Shipping Conditions

Blue ice

Storage Conditions

Anti-H-2K In Vivo Antibody - Low Endotoxin (Y-3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Specificity

Anti-H-2K In Vivo Antibody - Low Endotoxin (Y-3) recognizes/react with the mouse H-2Kb, H-2Kk, H-2Kq, H-2Kr, H-2ks and not H-2Kd MHC class I alloantigens

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Antigen Distribution

This Y-3 monoclonal antibody is reported to react with the mouse H-2Kb, H-2Kk, H-2Kq, H-2Kr, H-2ksand not H-2Kd MHC class I alloantigens.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

Mouse IgG2b Isotype Control for In Vivo - Low Endotoxin [MPC-11] (ICH2250)

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