Anti-Mouse TCR V Gamma 2 (UC3-10A6) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an alpha and beta chain in 95% of T cells, while 5% of T cells have TCRs consisting of gamma and delta chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.
Product Name Alternative
V gamma 2
Host
Armenian Hamster
Reactivity
Mouse
Target
TCR V gamma 2
Isotype
IgG
Clone
UC3-10A6
Applications
Flow Cytometry, Immunoprecipitation, In vivo Depletion
Purification Method
This monoclonal antibody was purified using Protein A
Concentration
1.0 - 5.0 mg/mL
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Shipping Conditions
Blue ice
Storage Conditions
Anti-TCR V gamma 2 In Vivo Antibody - Low Endotoxin (UC3-10A6) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Related Products
Anti-TCR V gamma 2 In Vivo Antibody - Ultra Low Endotoxin [UC3-10A6] (ICH1140UL)
Specificity
This antibody reacts with Vγ 2 T-Cell Receptor (TCR) -bearing T lymphocytes.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
V gamma 2 T-Cell Receptor (TCR) -bearing T lymphocytes make up a significant proportion of gamma delta TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 μg per 1.0 X 106 cells in a 100 μl total staining volume, followed by Goat Goat Anti-Armenian Hamster IgG (H&L) -R-phycoerythrin Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)
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